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Acetyl-Histone H3 (Lys9) Mouse Monoclonal Antibody
Description
Images & Validation
−| Tested Applications | WB |
|---|---|
| Dilution range | WB=1:500-2000 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | H3C6 |
| Target | H3C1 |
| Purification | Antigen affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: (-) HeLa, (+) HeLa + Sodium butyrate (30mM, 4hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 17 kDa, Observed MW: 17 kDa.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:500, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: mouse liver, mouse spleen, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 17 kDa, Observed MW: 17 kDa.
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Acetyl-Histone H3 (Lys9) Mouse Monoclonal Antibody (orb1172840)
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