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PCLO Antibody

SKU: orb154403

Description

Mouse monoclonal antibody to PCLO which is multidomain protein of the presynaptic cytomatrix in neurons, that is present in all vertebrate synapses, but is absent from invertebrates. PCLO may act as a scaffolding protein involved in the organization of synaptic active zone.

Images & Validation

Tested ApplicationsFC, WB
ReactivityHuman
Application Notes
Flow cytometry: Recommended dilution: 1-5 μg/ml, intracellular staining.Western blotting: A small splice variant of PCLO can be analyzed by Western blotting, however, the whole protein is too large (over 400 kDa) to be successfully processed by SDS PAAGE.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG1
Clone No.PCLO-01
ImmunogenHuman recombinant PCLO protein
TargetPCLO
PurificationPurified by protein-A affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

ACZ, PCH3, piccolo, aczonin

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PCLO Antibody

Western blotting of human REH erythroid cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb PCLO-01 and goat anti-mouse IgG (H+L)-HRP conjugate. The zone evidently corresponds to a small splice variant of the PCLO protein present in this type of cell; the major form (over 500 kDa) expressed in neurons is too large for analysis by Western blotting.

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol

PCLO Antibody (orb154403)

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Available Sizes

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0.1 mg
$ 360.00
DispatchUsually dispatched within 5-10 working days weeks.
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