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Anti-VISTA [13F3]
Description
Images & Validation
−| Tested Applications | Blocking, ELISA, FC |
|---|---|
| Reactivity | Mouse |
Key Properties
−| Host | Hamster |
|---|---|
| Clonality | Monoclonal |
| Isotype | Hamster IgG |
| Clone No. | 13F3 |
| Immunogen | This antibody was raised by immunising Armenian hamsters with EL4 cells overexpressing VISTA-RFP, and VISTA-Ig fusion protein. |
| Target | VISTA |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Binding curve of anti-VISTA antibody 13F3 (orb758883) to recombinant mouse VISTA Fc-Fusion Protein. ELISA Plate coated with recombinant mouse VISTA Fc-Fusion Protein at a concentration of 5 µg/ml. A 3-fold serial dilution from 10000 ng/ml was performed using orb758883. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.
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Protocol Information
Anti-VISTA [13F3] (orb758883)
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