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CD5 Antibody
SKU: orb2641426
Description
Images & Validation
−Item 1 of 4
| Tested Applications | FACS, IF, IHC-P, WB |
|---|---|
| Dilution range | Flow cytometry: 1-2ug/10^6 cells,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2b, kappa |
| Clone No. | CD5/2416 |
| Immunogen | A portion of amino acids 269-366 from the human protein was used as the immunogen for this CD5 antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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IHC staining of FFPE human tonsil with CD5 antibody (clone CD5/2416). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human Jurkat cell lysate with CD5 antibody (clone CD5/2416). Observed molecular weight: 55~67 kDa depending on glycosylation level.
Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using CD5 antibody (clone CD5/2416). These results demonstrate the foremost specificity of the CD5/2416 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free CD5 antibody (clone CD5/2416) as confirmation of integrity and purity.
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UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
CD5 Antibody (orb2641426)
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