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EphA1 Antibody (N-term)
SKU: orb1929106
Description
Images & Validation
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| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB1576 |
| Target | This EphA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-60 amino acids from the N-terminal region of human EphA1. |
| Molecular Weight | 108127 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−Ephrin type-A receptor 1, hEpha1, EPH tyrosine kinase, EPH tyrosine kinase 1, Erythropoietin-producing hepatoma receptor, Tyrosine-protein kinase receptor EPH, EPHA1, EPH, EPHT, EPHT1
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Western blot analysis of anti-EphA1 N-term Pab in HeLa cell lysate. EphA1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Flow cytometric analysis of HepG2 cells using EphA1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Quick Database Links
Gene Symbol
This EphA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-60 amino acids from the N-terminal region of human EphA1.
UniProt
RefSeq (Protein):NP_005223.4
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_005223.4 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
EphA1 Antibody (N-term) (orb1929106)
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