GLI2 Antibody

Link between metformin and Hedgehog signaling. (A) GLI1, GLI2 and PTCH1 gene expression after 72-h metformin treatment. Means ± SEM of two independent experiments. * p < 0.05 vs. control; (B) PTCH1, GLI1 and GLI2 protein expression after 72-h metformin treatment; (C) (p)AMPK protein and GLI1 expression in 22Rv1 cells transfected with AMPK siRNA and treated with metformin (5 mM) 72-h prior to protein lysis. GLI1, glioma-associated oncogene homolog 1; GLI2, glioma-associated oncogene homolog 2; PTCH1, patched 1.

Biorbyt's Affinity Purified anti-Gli2 antibody shows strong cytoplasmic and membranous staining of tumor cells in human breast tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.

Western blot using Biorbyt's affinity purified anti-Gli-2 antibody shows detection of Gli-2 protein. Lane 1: rat testes and Lane 2: human HEK293 (p/n orb348669) whole cell lysates (arrowhead). Each lane contains approximately 35 µg of lysate. Primary antibody was used at a 1:400 dilution in 5% BLOTTO (p/n orb348624) in PBS overnight at 4°C. The membrane was washed and reacted with a 1:10000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).