GLI/GLI1 Antibody

SKU: orb1535679

Description

GLI/GLI1 Antibody

Images & Validation

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Item 1 of 1

Tested Applications	IHC, IHC-P, WB
Dilution Range	IHC, IHC-P (10 µg/ml), WB (1:2000)
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	4E2
Immunogen	Human recombinant protein fragment corresponding to amino acids 755-1106 of human GLI1 (NP_005260) produced in E. coli.
Target	GLI / GLI1
Purification	Immunoaffinity purified from ascites
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% rAlbumin
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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GLI1, Gli-1, GLI family zinc finger 1, Glioma-associated oncogene, Glioma-associated oncogene 1, Oncogene GLI, GLI, Zinc finger protein GLI1

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI1.

Quick Database Links

Gene Symbol

GLI / GLI1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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