Goat Human IgG (H&L), F(ab)'2 fragment, ALP conjugated, min, cross-reactivity to bovine/mouse/rabbit serum Antibody

SKU: orb700325

Description

Goat polyclonal antibody against Goat anti-Human IgG (H&L) conjugated to AP

Images & Validation

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Tested Applications	ELISA, ICC, IHC, WB
Dilution range	1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Reactivity	Human
Application Notes	This antibody reacts with the heavy (gamma) chains on human IgG and light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins and serum from mouse, rabbit of bovine serum proteins based on immunoelectrophoresis. ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1% (w/v) BSA, Protease/IgG free. 0.05% (w/v) of sodium azide is added as preservative.Purity ≥90% based on SDS-PAGE. May contain small amounts of intact IgG Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Immunogen	Purified human IgG (H&L)
Purification	Immunogen affinity purified goat IgG.
Conjugation	ALP

Storage & Handling

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Storage	Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50% to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Form/Appearance	Liquid
Disclaimer	For research use only

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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