Guinea Pig Serum Antibody Peroxidase Conjugated

SKU: orb2652795

Description

Images & Validation

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Item 1 of 1

Tested Applications	DOT
Dilution range	ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Reactivity	Guinea pig
Application Notes	Anti-Guinea Pig Serum Peroxidase Conjugated has been tested by dot blot and is suitable to be assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 100 µL

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Guinea Pig serum proteins
Purity	Guinea Pig Serum is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum. Multiple precipitin arcs were observed against Guinea Pig Serum.
Conjugation	HRP

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1 mg/mL
Disclaimer	For research use only

Alternative Names

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Rabbit anti-Guinea Pig IgG F(ab')2 Fluorescein Conjugated Antibody, Rabbit anti-Guinea Pig IgG F(ab')2 FlTC Conjugated Antibody, Rabbit anti-Guinea Pig IgG Fab2 Fluorescein Conjugated Antibody, Rabbit anti-Guinea Pig IgG Fab2 FlTC Conjugated Antibody

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Guinea Pig Serum Antibody Peroxidase Conjugated

Dot Blot of Rabbit Anti-Guinea Pig Peroxidase Conjugated Antibody. Lane 1: 140 ng. Lane 2: 46 ng. Lane 3: 15.6 ng. Lane 4: 5.2 ng. Lane 5: 1.72 ng. Secondary Antibody: Rb-a-Guinea Pig HRP at 1 µg/ml. Blocking Buffer: BlockOut orb348644 for 30 min at RT.

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DOT

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