Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (M1R)

SKU: orb2975663

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Monkeypox virus M1R antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Human Anti-Monkeypox virus IgG (M1R) in the sample competitively binds to the pre-coated protein with biotin-labeled Human Anti-Monkeypox virus IgG (M1R). After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Human Anti-Monkeypox virus IgG (M1R) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Images & Validation

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Key Properties

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Sample Types	Plasma, Serum
Assay Type	Quantitative
Range	15.6 ng/mL - 1,000 ng/mL

Storage & Handling

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Storage	When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20°C, the rest reagents should be store at 4°C.
Disclaimer	For research use only

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