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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human MMP9 (611-707/707 aa) |
| Target | MMP9 |
| Molecular Weight | 78 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Matrix metalloproteinase-9 precursor; MMP-9; MMP9; MMP 9; 92 kDa type IV; Collagenase; 92 kDa gelatinase; Gelatinase B; GELB; MMP9_HUMAN; 82 kDa matrix metalloproteinase-9; 92 kDa type IV collagenase; CLG 4B; CLG-4B; CLG4B; Collagenase Type 4 beta; Collagenase Type-4 beta; Collagenase type IV 92 KD; Collagenase type IV 92 KD; EC 3.4.24.35; Gelatinase 92 KD; Gelatinase 92 KD; Gelatinase beta; Gelatinase-beta; GelatinaseB; GELB; Macrophage gelatinase; MANDP2; Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix Metalloproteinase 9; Type V collagenase.
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MMP9 Rabbit pAb [orb101739]
ELISA, IF, IHC-Fr, IHC-P
Canine, Equine, Mouse, Porcine, Rat
Human
Rabbit
Polyclonal
Unconjugated
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Sample: Lane 1: Human U2OS (TPA) cell lysates, Primary: Anti-MMP9 (orb11064) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 78 kDa, Observed band size: 85 kDa.
Tissue/Cell: Human laryngeal tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MMP9 Polyclonal Antibody, Unconjugated (orb11064) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: Human lung cancer tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citr Human lung cancerate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MMP9 Polyclonal Antibody, Unconjugated (orb11064) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MMP-9 Polyclonal Antibody, Unconjugated (orb11064) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Quick Database Links
Gene Symbol
MMP9
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Filter by Applications
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Jie Zhong 1 2, Yingzi Guo 1 2, Wang Xin EMMPRIN aggravates angiogenesis and blood-retina barrier injury by regulating matrix metalloproteinases in diabetic retinopathy Diab Vasc Dis Res ., (2025)
Applications
WB
Reactivity
Rat
Møller, Henrik Devitt et al. Role of fibulin-5 in metastatic organ colonization Mol Cancer Res, 9, 553-563 (2011)
Applications
IHC
Reactivity
Mouse
Buile, Dace et al. Evaluation of the Multiple Tissue Factors in the Cartilage of Primary and Secondary Rhinoplasty in Cleft Lip and Palate Patients Pediatr Rep, 14, 419-433 (2022)
Applications
IHC
Reactivity
Human
MMP9 Rabbit pAb (orb11064)
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