Neurofilament Antibody Cocktail

SKU: orb2639664

Description

This mAb reacts with a 200kDa and 68kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

Images & Validation

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Tested Applications	FACS, IHC-P
Dilution range	Flow cytometry: 0.5-1ug/10^6 cells,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Reactivity	Human, Mouse, Rat
Application Notes	Optimal dilution of the Neurofilament antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	RT-97 + NR-4
Immunogen	Triton-X 100 insoluble proteins of rat brain (RT-97) and Neurofilaments from porcine spinal cord (NR-4) were used as the immunogen for the Neurofilament antibody cocktail.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC: Formalin-fixed, paraffin-embedded human cerebellum stained with Neurofilament antibody (RT-97 + NR-4).

IHC: Formalin-fixed, paraffin-embedded rat cerebellum stained with Neurofilament antibody (RT-97 + NR-4).

Flow cytometry testing of permeabilized human HEK293 cells with Neurofilament antibody cocktail (clone RT-97 + NR-4); Red = isotype control, Blue = Neurofilament antibody cocktail.

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UniProt

P12036

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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