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Rabbit BCKDHA, Phospho (S292) Antibody
SKU: orb1522551
Description
Research Area
Metabolism Research
Images & Validation
−Item 1 of 3
| Tested Applications | WB |
|---|---|
| Dilution range | WB - 1:10,000 - 1:25,000; IP - Not recommended |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Surrounding serine 292 |
| Target | BCKDHA Phospho (S292) |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−2-oxoisovalerate dehydrogenase (lipoamide); 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; BCKDE1A; BCKDH E1-alpha; branched chain keto acid dehydrogenase E1 alpha protein; branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; MSU; MSUD1; OVD1A
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Detection of mouse pBCKDHA (S292) by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-pBCKDHA (S292) antibody (orb1522551) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human pBCKDHA (S292) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells treated with phosphatase (+) or mock treated (-). Antibody: Affinity purified rabbit anti-pBCKDHA (S292) antibody (orb1522551) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-BCKDHA antibody.
Detection of human pBCKDHA (S292) by western blot. Samples: Whole cell lysate (10 µg) from Hep-G2, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-pBCKDHA (S292) antibody (orb1522551) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000700.1 |
|---|
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Rabbit BCKDHA, Phospho (S292) Antibody (orb1522551)
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