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Rabbit anti-GTF2H1 Antibody
SKU: orb1524688
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 250 and 300 |
| Target | GTF2H1 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−general transcription factor IIH polypeptide 1; TFIIH; TFB1; P62; general transcription factor IIH subunit 1; TFIIH basal transcription factor complex p62 subunit; epididymis secretory sperm binding protein; BTF2 p62; BTF2; basic transcription factor 2 62 kDa subunit; general transcription factor IIH, polypeptide 1, 62kDa
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Detection of human GTF2H1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , Jurkat (J; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-GTF2H1 antibody orb1524688 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. GTF2H1 was also immunoprecipitated by rabbit anti-GTF2H1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Detection of human GTF2H1 by immunohistochemistry.Sample: FFPE section of human testis. Antibody: Affinity purified rabbit anti-GTF2H1 (Cat. No. orb1524688) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001135779.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit anti-GTF2H1 Antibody (orb1524688)
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