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Rabbit anti-SNRNP200 Antibody
SKU: orb1524806
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 400 and 450 |
| Target | SNRNP200 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−BRR2 homolog; small nuclear ribonucleoprotein 200kDa (U5); U5-200KD; U5 snRNP-specific 200 kDa protein; U5 small nuclear ribonucleoprotein 200 kDa helicase; small nuclear ribonucleoprotein, U5 200kDa subunit; HELIC2; activating signal cointegrator 1 complex subunit 3-like 1; BRR2; bad response to refrigeration 2 homolog; ASCC3L1; RP33
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Detection of human SNRNP200 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, K-562, Jurkat, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SNRNP200 antibody (orb1524806) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of mouse SNRNP200 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SNRNP200 antibody (orb1524806) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human SNRNP200 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SNRNP200 antibody (orb1524806) used for IP at 6 µg per reaction. SNRNP200 was also immunoprecipitated by a previous lot of this antibody (orb1524806) and a second antibody against a different epitope of SNRNP200.
Detection of human SNRNP200 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- SNRNP200 (Cat. No. orb1524806) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_054733.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit anti-SNRNP200 Antibody (orb1524806)
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