RecombinantMPIF/CCL23(aa46-120),Human

SKU: orb1494678

Description

Myeloid progenitor inhibitory factor 1 (MPIF-1), also known as Chemokine (C-C motif) ligand 23 (CCL23) is a small cytokine belonging to the CC chemokine family. MPIF-1 is predominantly expressed in lung and liver tissue, but is also found in bone marrow and placenta. It is also expressed in some cell lines of myeloid origin. It is highly chemotactic for resting T cells and monocytes and slightly chemotactic for neutrophils. MPIF-1 has been shown to inhibit colony formation of bone marrow myeloid immature progenitors. It has also been attributed to an inhibitory activity on hematopoietic progenitor cells. MPIF-1 is a ligand for the chemokine receptor CCR1. Recombinant human MPIF-1/CCL23 (aa46-120) produced in CHO cells is a single polypeptide chain containing 75 amino acids. A fully biologically active molecule, rhMPIF-1/CCL23 (aa46-120) has a molecular mass of 11.3 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O or PBS at 100 μg/ml.

Key Properties

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Source	CHO
Biological Activity	The EC50 value of human MPIF-1/CCL23 (aa46-120) on Caˆ2+ mobilization assay in CHO-K1/ Gα15/hCCR1 cells (human Gα15 and human CCR1 stably expressed in CHO-K1 cells) is less than 1 μg/ml.
Molecular Weight	11.3 kDa, observed by reducing SDS-PAGE.
Protein Sequence	RFHATSADCCISYTPRSIPCSLLESYFETNSECSKPGVIFLTKKGRRFCANPSDKQVQVCVRMLKLDTRIKTRKN
Purification	> 95% as analyzed by SDS-PAGE.
Purity	> 95% as analyzed by SDS-PAGE.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant Human MPIF/CCL23 (aa46-120) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Human MPIF/CCL23 (aa46-120) should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against PBS.
Buffer/Preservatives	Lyophilized after extensive dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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MPIF-1, CCL23

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