14-3-3 zeta+delta Rabbit Polyclonal Antibody

SKU: orb100007

This product can be custom made

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Description

14-3-3 zeta+delta Rabbit Polyclonal Antibody

Research Area

Alzheimer's Disease, Cancer Research, Cell Biology, Cytoplasmic, Neurology, Neuroscience, Signal Transduction, Synapse Markers, Tumor Biology

Images & Validation

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Item 1 of 4

Tested Applications	FC, WB
Dilution Range	WB=1:500-2000, Flow-Cyt=1ug/Test
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Equine, Gallus, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human 14-3-3 zeta/delta (51-150/245aa)
Target	YWHAZ
Molecular Weight	27 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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GW128; HEL-S-1; HS1; KCIP-1; YWHAA; 14-3-3-zeta; HEL-S-3; HEL-S-93; HEL4; POPCHAS; YWHAD; 1110013I11Rik; 14-3-3zeta; 1300003C17Rik; 14-3-3b; 14-3-3z; 143B_BOVIN; 1433B_HUMAN; YWHAB; Protein 1054; Protein kinase C inhibitor protein 1 (KCIP-1); 1433Z_HUMAN; YWHAZ; 143Z_MOUSE; 1433B_MOUSE; 1433B_RAT; Prepronerve growth factor RNH-1; 1433Z_RAT; Mitochondrial import stimulation factor S1 subunit; Msfs1; 1433Z_SHEEP;

Quality Guarantee

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14-3-3 zeta+delta Rabbit Polyclonal Antibody

Blank control (black line): Raji (black) (The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with PBST for 30 min on room temperature), Primary Antibody (rad line): Rabbit Anti-14-3-3 zeta+delta antibody (orb100007), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (grenn line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/test.

Blank control (blue line): HL60 (fixed with 70% ethanol (overninght at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-14-3-3 zeta+delta antibody (orb100007), Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-14-3-3 zeta+delta (orb100007) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.

Sample: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-14-3-3 zeta+delta (orb100007) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.

Quick Database Links

Gene Symbol

YWHAZ

UniProt

P31946

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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