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14-3-3 zeta+delta Rabbit Polyclonal Antibody
SKU: orb100007
Featured
Description
Research Area
Alzheimer's Disease, Cancer Research, Cell Biology, Cytoplasmic, Neurology, Neuroscience, Signal Transduction, Synapse Markers, Tumor Biology
Images & Validation
−Item 1 of 4
| Tested Applications | FC, WB |
|---|---|
| Dilution range | WB=1:500-2000, Flow-Cyt=1ug/Test |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Gallus, Rabbit |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human 14-3-3 zeta/delta (51-150/245aa) |
| Target | YWHAZ |
| Molecular Weight | 27 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−14 3 3 protein delta; 14 3 3 protein zeta/delta; 14 3 3 protein/cytosolic phospholipase A2; 14-3-3 protein zeta/delta; 14-3-3zeta; 14-3-3 delta; 14-3-3 zeta; 14-3-3delta; 1433Z_HUMAN; KCIP 1; KCIP-1; KCIP1; MGC111427; MGC126532; MGC13815; phospholipase A2; Protein kinase C inhibitor protein 1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein zeta polypeptide; YWHAZ.
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Blank control (black line): Raji (black) (The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with PBST for 30 min on room temperature), Primary Antibody (rad line): Rabbit Anti-14-3-3 zeta+delta antibody (orb100007), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (grenn line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/test.
Blank control (blue line): HL60 (fixed with 70% ethanol (overninght at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-14-3-3 zeta+delta antibody (orb100007), Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE.
Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-14-3-3 zeta+delta (orb100007) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.
Sample: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-14-3-3 zeta+delta (orb100007) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.
Quick Database Links
Gene Symbol
YWHAZ
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
14-3-3 zeta+delta Rabbit Polyclonal Antibody (orb100007)
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