ADAR1 Rabbit Polyclonal Antibody

SKU: orb13237

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Description

Images & Validation

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Item 1 of 4

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human DRADA (1001-1226/1226aa)
Target	ADAR
Molecular Weight	135 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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136kDa double stranded RNA binding protein; Adar 1; ADAR; Adar1; Adenosine deaminase RNA specific 1; Adenosine deaminase RNA specific; Adenosine deaminase that act on RNA; AV242451; Double stranded RNA specific adenosine deaminase; Double-stranded RNA-specific editase Adar; Drada; Dsh; Dsrad; dsRNA adenosine deaminase; EC 3.5.4.-; G1P1; IFI 4; IFI4; Ifi4 protein; Interferon induced protein 4; Interferon inducible protein 4; K88dsrbp; mZaADAR; p136; Pre-mRNA adenosine deaminase; RNA adenosine deaminase 1; RNA-editing deaminase 1; RNA-editing enzyme 1.

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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ADAR1) Polyclonal Antibody, Unconjugated (orb13237) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Sample: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-ADAR1 (orb13237) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 135 kD, Observed band size: 110 kD.

Sample: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-ADAR1 (orb13237) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 135 kD, Observed band size: 110 kD.

Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-ADAR1 (orb13237) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 135 kD, Observed band size: 110 kD.

Quick Database Links

Gene Symbol

ADAR

UniProt

P55265

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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