Adiponectin Rabbit Polyclonal Antibody

SKU: orb10048

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Description

Research Area

Adipogenesis, Cardiovascular Research, Diabetes Research, Hormone Research, Hypoxia, Metabolism, Obesity, cell Biology

Images & Validation

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Item 1 of 5

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Gallus, Porcine, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Adiponectin (151-244/244aa)
Target	ADIPOQ
Molecular Weight	25 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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30 kDa adipocyte complement related protein; 30 kDa adipocyte complement-related protein; ACDC; ACRP 30; ACRP30; ADIPO_HUMAN; Adipocyte; Adipocyte C1q and collagen domain containing protein; Adipocyte complement related 30 kDa protein; Adipocyte complement related protein of 30 kDa; Adipocyte complement-related 30 kDa protein; Adiponectin; AdipoQ; Adipose most abundant gene transcript 1; Adipose most abundant gene transcript 1 protein; Adipose specific collagen like factor; ADIPQTL1; ADPN; APM 1; apM-1; ApM1; C1q and collagen domain-containing protein; GBP 28; GBP28; Gelatin binding protein; Gelatin binding protein 28; Gelatin-binding protein.

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Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Adiponectin (orb10048) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 25 kD, Observed band size: 27 kD.

Sample: Liver (Rat) Lysate at 40 ug, Intestine (Mouse) Lysate at 40 ug, Primary: Anti-Adiponectin (orb10048) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 25 kD, Observed band size: 25 kD.

Sample: muscle (Mouse) Lysate at 40 ug, Primary: Anti-Adiponectin (orb10048) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 30 kD.

Tissue/cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Adiponectin Polyclonal Antibody, Unconjugated (orb10048) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: mouse liver tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Adiponectin Polyclonal Antibody, Unconjugated (orb10048) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

ADIPOQ

UniProt

Q15848

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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