AF10 Rabbit Polyclonal Antibody

SKU: orb5676

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Description

Images & Validation

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Tested Applications	FC, WB
Dilution Range	WB=1:500-2000, Flow-Cyt=1μg /Test
Reactivity	Human, Rat
Predicted Reactivity	Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human AF10/MLLT10 (151-250/1027aa)
Target	MLLT10
Molecular Weight	109 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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AF 10; AF10; AF10_HUMAN; ALL 1 fused gene from chromosome 10; ALL1 fused gene from chromosome 10; ALL1-fused gene from chromosome 10 protein; MLLT 10; MLLT10; Myeloid/lymphoid or mixed lineage leukemia(trithorax homolog Drosophila) translocated to 10; Myeloid/lymphoid or mixed lineage leukemia translocated to 10; Protein AF 10; Protein AF-10; Protein AF10; Type I AF10 protein.

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Blank control: U-2OS (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:100 in 1X PBS containing 0.5% BSA, Primary Antibody, dilution: 1 µl in 100 µl 1X PBS containing 0.5% BSA (green).

Sample: Testis (Rat) Lysate at 40 ug, Primary: Anti-AF10 (orb5676) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 109 kD, Observed band size: 109 kD.

Quick Database Links

Gene Symbol

MLLT10

UniProt

P55197

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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