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Description
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−Item 1 of 4
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | WB=1:500-2000, ELISA=1:5000-10000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant human Alpha-internexin protein (1-499/499aa) |
| Target | INA |
| Molecular Weight | 60-66 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−NEF5; NF-66; NF66; TXBP-1; alpha-Inx; Inexa; Intlaa; AINX_HUMAN; INA; 66 kDa neurofilament protein (NF-66 | Neurofilament-66); Neurofilament 5; AINX_MOUSE; AINX_RAT; internexin neuronal intermediate filament protein alpha; internexin neuronal intermediate filament protein, alpha
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Measured by its binding ability in a indirect ELISA. Immobilized Human alpha Internexin protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human alpha Internexin Antibody, the EC50 is 312.5 ng/ml.
Measured by its binding ability in a indirect ELISA. Immobilized Human alpha Internexin protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human alpha Internexin Antibody, the EC50 is 911.2 ng/ml.
Sample: Lane 1: Human SH-SY5Y cell lysates, Lane 2: Human SH-SY5Y cell lysates, Primary: Anti-alpha Internexin (orb1291524) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kDa, Observed band size: 66 kDa.
Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Primary: Anti-alpha Internexin (orb1291524) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kDa, Observed band size: 60 kDa.
Quick Database Links
Gene Symbol
INA
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Alpha Internexin Rabbit Polyclonal Antibody (orb1291524)
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