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Description
Research Area
Musculoskeletal & Connective Tissue Research
Images & Validation
−
Item 1 of 13
| Tested Applications | FC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Rabbit |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Actin alpha (301-375/375aa) |
| Target | ACTA2 |
| Molecular Weight | 42 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ACTSA; SMDYS; 0610041G09Rik; Actvs; SMAalpha; SMalphaA; a-SMA; alphaSMA; ACT-4; actin; ACTA_BOVIN; ACTA2; Alpha-actin-2; 3.6.4.-; ACTA_HUMAN; Cell growth-inhibiting gene 46 protein; ACTA_MOUSE; ACTA_RABIT; ACTA_RAT; actin alpha 2, smooth muscle; actin, alpha 2, smooth muscle, aorta
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Quick Database Links
Gene Symbol
ACTA2
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
Filter by Applications
Filter by Species
Zeynab Yousefi 1, Mitra Nourbakhsh 2, Abbas Sahebghadam Lotfi Pirfenidone Downregulates eIF6, P311, and TGF-β Expression and Improves Liver Fibrosis Induced by Bile Duct Ligation in Wistar Rats: Evidence for Liver Regeneration DNA Cell Biol, (2024)
Applications
IHC
Reactivity
Rat
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