AMPK alpha-1 Mouse Monoclonal Antibody

SKU: orb499641

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Description

Research Area

Alzheimer's disease, Cancer Research, Cell Biology, Energy Metabolism, Fatty Acid Oxidation, Fatty Acids, Hypoxia, Kinases, Metabolism, Neurodegenerative Disease, Neuroscience

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-1000, IHC-P=1:200-800, IHC-F=1:200-500, IF=1:200-500
Reactivity	Mouse, Rat
Predicted Reactivity	Human

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	11G3
Immunogen	Recombinant human AMPK alpha-1 Protein
Target	PRKAA1
Molecular Weight	64 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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AMPK alpha 1; 5 AMP activated protein kinase alpha 1catalytic subunit; 5 AMP activated protein kinase catalytic alpha 1 chain; 5' AMP activated protein kinase catalytic subunit alpha 1; AAPK1; acetyl CoA carboxylase kinase; AI194361; AI450832; AL024255; AMP-activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK 1; AMPK alpha 1 chain; AMPK; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; cb116; EC 2.7.11.1; HMG CoA reductase kinase; hormone sensitive lipase kinase; im:7154392; kinase AMPK alpha1; MGC33776; MGC57364; PRKAA 1; PRKAA1; Protein kinase AMP activated alpha 1 catalytic subunit; SNF1-like protein AMPK; wu:fa94c10; AAPK1_HUMAN; AMPKα1; AMPK α1; AMPKα 1; AMPK α 1; AMPK AMPK-α1; AMPK-α-1; α-1; AMPK a1.

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Paraformaldehyde-fixed, paraffin embedded (Mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (AMPK alpha-1) Monoclonal Antibody, Unconjugated (orb499641) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Rat Heart Lysates, Lane 2: Rat Brain Lysates, Lane 3: Rat Skeletal muscle Lysates, Primary: Anti-AMPK alpha-1 (orb499641) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 64kDa, Observed band size: 64kDa.

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Gene Symbol

PRKAA1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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