Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E.coli-derived human ACSL3 recombinant protein (Position: M12-I224). Human ACSL3 shares 91.1% and 90.1% amino acid (aa) sequence identity with mouse and rat ACSL3, respectively.
Target	Fatty acid CoA ligase Acsl3
Molecular Weight	80 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 5mg rAlbumin, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Long-chain-fatty-acid--CoA ligase 3; 6.2.1.3; Long-chain acyl-CoA synthetase 3; LACS 3; ACSL3; ACS3, FACL3, LACS3

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Western blot analysis of ACSL3 using anti-ACSL3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. lane 1: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACSL3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACSL3 at approximately 80KD. The expected band size for ACSL3 is at 80KD.

Quick Database Links

Gene Symbol

Fatty acid CoA ligase Acsl3

UniProt

O95573

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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