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BID Antibody
SKU: orb182359
Description
Research Area
Cardiovascular Research, Immunology & Inflammation, Metabolism Research
Images & Validation
−Item 1 of 7
| Tested Applications | FC, ICC, IF, IHC, WB |
|---|---|
| Dilution range | Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequences identity with mouse and rat Bid, respectively. |
| Target | BH3-interacting domain death agonist |
| Molecular Weight | 22 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 5mg rAlbumin, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Disclaimer | For research use only |
Alternative Names
−Antigen KI-67; MKI67
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Flow Cytometry analysis of MCF-7 cells using anti-BID antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of BID using anti-BID antibody.Lane 1:HELA Cell;2:COLO320 Cell;3:JURKAT Cell;4:SKOV Cell.
IF analysis of Bid using anti-Bid antibody. Bid was detected in immunocytochemical section of MCF-7 cells.
IHC analysis of BID using anti-BID antibody.BID was detected in paraffin-embedded section of human mammary cancer tissues.
IHC analysis of BID using anti-BID antibody.BID was detected in paraffin-embedded section of mouse spleen tissues.
IHC analysis of BID using anti-BID antibody.BID was detected in paraffin-embedded section of rat intestine tissues.
IHC analysis of BID using anti-BID antibody.BID was detected in immunocytochemical section of A549 cell.
Quick Database Links
Gene Symbol
BH3-interacting domain death agonist
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
BID Antibody (orb182359)
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