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BID Antibody
Description
Images & Validation
−| Tested Applications | FC, ICC, IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequences identity with mouse and rat Bid, respectively. |
| Molecular Weight | 22 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Disclaimer | For research use only |
Alternative Names
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100 μgBid Rabbit Polyclonal Antibody [orb10188]
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200 μl, 100 μl, 50 μl

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Flow Cytometry analysis of MCF-7 cells using anti-BID antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of BID using anti-BID antibody.Lane 1:HELA Cell;2:COLO320 Cell;3:JURKAT Cell;4:SKOV Cell.

IF analysis of Bid using anti-Bid antibody. Bid was detected in immunocytochemical section of MCF-7 cells.

IHC analysis of BID using anti-BID antibody.BID was detected in paraffin-embedded section of human mammary cancer tissues.

IHC analysis of BID using anti-BID antibody.BID was detected in paraffin-embedded section of mouse spleen tissues.

IHC analysis of BID using anti-BID antibody.BID was detected in paraffin-embedded section of rat intestine tissues.

IHC analysis of BID using anti-BID antibody.BID was detected in immunocytochemical section of A549 cell.
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UniProt Details
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Protocol Information
BID Antibody (orb182359)
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