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CD20 Antibody
SKU: orb1806379
Description
Images & Validation
−Item 1 of 6
| Tested Applications | ELISA, FA, FACS, In vivo |
|---|---|
| Reactivity | Human, Monkey |
Key Properties
−| Clonality | Monoclonal |
|---|---|
| Target | CD20 |
| Source/Expression System | CHO |
| Molecular Weight | 145.2 kDa |
| Purity | >95% |
| Purification | Protein A |
| Endotoxin | <0.001 EU/ug |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100 mM Proline/Acetic acid 20mM Arginine pH 5.0 |
| Concentration | Batch dependent |
| Disclaimer | For research use only |
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Anti-CD20 Reference Antibody (rituximab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Anti-CD20 Reference Antibody (rituximab)is more than 98.97% ;determined by SEC-HPLC.
Immobilized human EGFR His at 2 μg/mL can bind Anti-CD20 Reference Antibody (rituximab);EC50=0.01573/0.01258μg/mL.
Raji cells were stained with Anti-CD20 Reference Antibody (rituximab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC87=1.334 μg/mL.
Anti-CD20 Reference Antibody (rituximab)-induced CDC activity was evaluated using Raji Cell. The max Lysis rate was approximately 74%.
Anti-CD20 Reference Antibody (rituximab)-induced ADCC activity was evaluated using Raji Cell. The max Lysis rate was approximately 35%.
Quick Database Links
Gene Symbol
CD20
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD20 Antibody (orb1806379)
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