CD20/MS4A1 Rabbit Polyclonal Antibody

SKU: orb182382

This product can be custom made

Description

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 6

Tested Applications	FC, ICC, IF, IHC, IHC-Fr, WB
Dilution Range	Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6cells
Reactivity	Human

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E.coli-derived human CD20 recombinant protein (Position: M1-D261). Human CD20 shares 75% amino acid (aa) sequence identity with mouse CD20.
Target	B-lymphocyte antigen CD20
Molecular Weight	35 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD20; MS4A1; CD20

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Flow Cytometry analysis of U937 cells using anti-CD20 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of A431 cells using anti-CD20 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CD20 using anti-CD20 antibody.Lane 1:human Daudi cell.

IF analysis of CD20 using anti-CD20 antibody.CD20 was detected in paraffin-embedded section of human B lymphocytic tumor tissues.

IHC analysis of CD20 using anti-CD20 antibody.CD20 was detected in paraffin-embedded section of Human Tonsil Tissue.

Quick Database Links

Gene Symbol

B-lymphocyte antigen CD20

UniProt

P11836

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-Fr

Immunohistochemistry Frozen

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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