CD23/FCER2 Rabbit Polyclonal Antibody

SKU: orb182383

Description

Research Area

Immunology & Inflammation, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 11

Tested Applications	ELISA, FC, ICC, IF, IHC, IHC-Fr, WB
Dilution Range	Western blot, 0.1-0.5μg/ml, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry, 0.5-1μg/ml, Human Immunofluorescence, 2μg/ml, Mouse, Rat Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Mouse ELISA (Cap), 1-5μg/ml, Mouse
Reactivity	Human, Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E.coli-derived mouse CD23 recombinant protein (Position: E50-P331). Mouse CD23 shares 52% amino acid (aa) sequence identity with human CD23.
Target	Low affinity immunoglobulin epsilon Fc receptor
Molecular Weight	49 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Low affinity immunoglobulin epsilon Fc receptor; Fc-epsilon-RII; Lymphocyte IgE receptor; CD23; Fcer2; Fcer2a

Quality Guarantee

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Flow Cytometry analysis of mouse PBMC cells using anti-CD23 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of mouse BMC cells using anti-CD23 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CD23 using anti-CD23 antibody.Lane 1:Mouse Liver Tissue.

WB analysis of CD23 using anti-CD23 antibody.Lane 1:rat spleen tissue;2:rat thymus tissue.

IF analysis of CD23 using anti-CD23 antibody.CD23 was detected in paraffin-embedded section of mouse lymphaden tissues.

IF analysis of CD23 using anti-CD23 antibody.CD23 was detected in paraffin-embedded section of rat lymphaden tissues.

Sandwich ELISA - Recombinant mouse CD23/FCER2 protein standard curve.Use in combination with reagents from Mouse CD23/FCER2 ELISA Kit EZ-Set (DIY Antibody Pairs).

IHC analysis of CD23 using anti-CD23 antibody.CD23 was detected in paraffin-embedded section of Human Tonsil Tissue.

IHC analysis of CD23 using anti-CD23 antibody.CD23 was detected in paraffin-embedded section of Mouse Spleen Tissue.

IHC analysis of CD23 using anti-CD23 antibody.CD23 was detected in paraffin-embedded section of Rat Spleen Tissue.

IHC analysis of CD23 using anti-CD23 antibody.CD23 was detected in frozen section of mouse spleen tissues.

Quick Database Links

Gene Symbol

Low affinity immunoglobulin epsilon Fc receptor

UniProt

P20693

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-Fr

Immunohistochemistry Frozen

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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