CD44 antigen CD44 Rabbit Polyclonal Antibody

SKU: orb443085

Description

Research Area

Cancer Biology, Cell Biology, Disease Biomarkers, Immunology & Inflammation, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 5

Tested Applications	FC, IHC, WB
Dilution Range	Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
Reactivity	Human, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human CD44, different from the related mouse and rat sequences by one amino acid.
Target	CD44 antigen
Molecular Weight	82 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD44 antigen; CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I; PGP-I; CD44; CD44; LHR, MDU2, MDU3, MIC4

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CD44 antigen CD44 Rabbit Polyclonal Antibody

Flow Cytometry analysis of HL-60 cells using anti-CD44 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of CD44 using anti-CD44 antibody.Lane 1:human HeLa cell;2:rat PC-12 cell.

IHC analysis of CD44 using anti-CD44 antibody.CD44 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue.

IHC analysis of CD44 using anti-CD44 antibody.CD44 was detected in a paraffin-embedded section of human rectal cancer tissue.

IHC analysis of CD44 using anti-CD44 antibody.CD44 was detected in a paraffin-embedded section of rat lung tissue.

Quick Database Links

Gene Symbol

CD44 antigen

UniProt

P16070

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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