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Description
Research Area
Autophagic, Cancer, Epigenetics, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, FC, ICC, IP, WB |
|---|---|
| Reactivity | Bovine, Human, Mouse, Porcine, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgM |
| Clone No. | BF-06 |
| Target | Clathrin Heavy Chain |
| Purification | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CHC, CLHC, MRD56
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Immunocytochemistry staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Immunocytochemistry staining of clathrin in human HeLa cell line using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Separation of MCF-7 cells stained using anti-Clathrin (BF-06) purified antibody (concentration in sample 2 µg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
Clathrin Heavy Chain Antibody (orb43680)
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