Claudin 5/CLDN5 Rabbit Polyclonal Antibody

SKU: orb669073

Description

Anti-Claudin 5/CLDN5 Antibody. Tested in IF, IHC applications. This antibody reacts with Human.

Research Area

Cardiovascular Research, Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	IF, IHC
Dilution Range	Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human Immunofluorescence, 5μg/ml, Human
Reactivity	Human

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human Claudin 5/CLDN5, which shares 95% and 90% amino acid (aa) sequence identity with mouse and rat Claudin 5/CLDN5, respectively.
Target	Claudin-5
Molecular Weight	130 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Concentration	500 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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AWAL; BEC1; claudin 5; claudin5; CLDN5; CPETRL1; TMDVCF; TMVCF

Quality Guarantee

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Claudin 5/CLDN5 Rabbit Polyclonal Antibody

IF analysis of Claudin 5/CLDN5 using anti-Claudin 5/CLDN5 antibody. Claudin 5/CLDN5 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-Claudin 5/CLDN5 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IHC analysis of Claudin 5/CLDN5 using anti-Claudin 5/CLDN5 antibody. Claudin 5/CLDN5 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Claudin 5/CLDN5 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Quick Database Links

Gene Symbol

Claudin-5

UniProt

O00501

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

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Immunofluorescence

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