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Cyclin D1/CCND1 Rabbit Polyclonal Antibody
SKU: orb180681
Description
Research Area
Cancer Biology, Cell Biology, Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | Western blot, 0.1-0.5μg/ml, Human, Rat Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human |
| Reactivity | Human, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Cyclin D1, different from the related mouse and rat sequences by two amino acids. |
| Target | G1/S-specific cyclin-D1 |
| Molecular Weight | 33 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 5mg rAlbumin, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Disclaimer | For research use only |
Alternative Names
−G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene; CCND1; BCL1, PRAD1
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Flow Cytometry analysis of U-87MG cells using anti-Cyclin D1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of Cyclin D1 using anti-Cyclin D1 antibody.Lane 1:Rat Testis Tissue; 2:Human Placenta Tissue; 3:Rat Brain Tissue; 4:MCF-7 Cell; 5:COLO320 Cell; 6:SW620 Cell; 7:MM231 Cell.
Quick Database Links
Gene Symbol
G1/S-specific cyclin-D1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
Cyclin D1/CCND1 Rabbit Polyclonal Antibody (orb180681)
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