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Fibroblast growth factor 2 FGF2 Rabbit Polyclonal Antibody
SKU: orb48039
Description
Research Area
Cancer Biology, Cardiovascular Research, Neuroscience, Signal Transduction, Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, FC, ICC, IF, IHC, WB |
|---|---|
| Dilution Range | Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry(Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5μg/ml, - |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human FGF2 recombinant protein (Position: P143-S288). |
| Target | Insulin-like growth factor 3 |
| Molecular Weight | 17-21 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Disclaimer | For research use only |
Alternative Names
−Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB
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Flow Cytometry analysis of SiHa cells using anti-FGF2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of FGF2 using anti-FGF2 antibody.Lane 1:human HeLa cell;2:human SKOV3 cell;3:human SiHa cell; 4:human u87 cell.
IF analysis of FGF2 using anti-FGF2 antibody. FGF2 was detected in an immunocytochemical section of SiHa cells.
Quick Database Links
Gene Symbol
Insulin-like growth factor 3
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Fibroblast growth factor 2 FGF2 Rabbit Polyclonal Antibody (orb48039)
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