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GFAP Rabbit Polyclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, Pig Immunofluorescence, 5μg/ml, Rat |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human GFAP recombinant protein (Position: Q93-M432). Human GFAP shares 94% amino acid (aa) sequence identity with both mouse and rat GFAP. |
| Target | Glial fibrillary acidic protein |
| Molecular Weight | 50 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 5mg rAlbumin, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Disclaimer | For research use only |
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WB analysis of GFAP using anti-GFAP antibody.Lane 1:human U251 cell; 2:human HeLa cell; 3:rat brain tissue; 4:rat C5 cell; 5:mouse brain tissue.

IF analysis of GFAP using anti-GFAP antibody.GFAP was detected in paraffin-embedded section of mouse brain tissues.

IHC analysis of GFAP using anti-GFAP antibody.GFAP was detected in paraffin-embedded section of Mouse Brain Tissue.

IHC analysis of GFAP using anti-GFAP antibody.GFAP was detected in paraffin-embedded section of Rat Brain Tissue.

IHC analysis of GFAP using anti-GFAP antibody.GFAP was detected in paraffin-embedded section of Human meningioma Tissue.
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GFAP Rabbit Polyclonal Antibody (orb196252)
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