Hsp90 alpha/HSP90AA1 Rabbit Polyclonal Antibody

SKU: orb196259

Description

Research Area

Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

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Item 1 of 8

Tested Applications	FC, ICC, IF, IHC, WB
Dilution Range	Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry , 0.5-1μg/ml, Human, - Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
Reactivity	Human, Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E.coli-derived human Hsp90 alpha recombinant protein (Position: P2-V365). Human Hsp90 alpha shares 99% amino acid (aa) sequence identity with both mouse and rat Hsp90 alpha.
Target	Heat shock protein HSP 90-alpha
Molecular Weight	95 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 5mg rAlbumin, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Heat shock protein HSP 90-alpha; Heat shock 86 kDa; HSP 86; HSP86; Lipopolysaccharide-associated protein 2; LAP-2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38; HSP90AA1; HSP90A, HSPC1, HSPCA

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Hsp90 alpha/HSP90AA1 Rabbit Polyclonal Antibody

ICC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody.Hsp90 Alpha was detected in an immunocytochemical section of A549 cells. Biotinylated goat anti-rabbit IgG was used as secondary antibody.

ICC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody.Hsp90 Alpha was detected in an immunocytochemical section of MCF-7 cells. Biotinylated goat anti-rabbit IgG was used as secondary antibody.

Flow Cytometry analysis of A549 cells using anti-Hsp90 alpha antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody.Lane 1:human HeLa cell; 2:human Jurkat cell; 3:human A431 cell; 4:rat brain tissue; 5:rat heart tissue; 6:mouse brain tissue; 7:mouse heart tissue.

IF analysis of Hsp90 alpha using anti-Hsp90 alpha antibody. Hsp90 alpha was detected in immunocytochemical section of MCF-7 cells.

IHC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody. Hsp90 Alpha was detected in a paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody. Hsp90 Alpha was detected in a paraffin-embedded section of mouse brain tissue.

IHC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody. Hsp90 Alpha was detected in a paraffin-embedded section of rat brain tissue.

Quick Database Links

Gene Symbol

Heat shock protein HSP 90-alpha

UniProt

P07900

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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