IL-17A/Il17a/IL Rabbit Polyclonal Antibody

SKU: orb865532

Description

Anti-IL-17A/Il17a Antibody. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC
Dilution Range	Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat ELISA, 0.1-0.5 μg/ml, Rat
Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E.coli-derived rat IL-17A/Il17a recombinant protein (Position: A18-S150).
Target	m7GpppN-mRNA hydrolase
Molecular Weight	27 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Concentration	500 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CTLA 8; CTLA8; IL 17; IL 17A; IL17; IL-17; IL17A; IL-17A; interleukin 17A

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IL-17A/Il17a/IL Rabbit Polyclonal Antibody

IHC analysis of IL-17A/Il17a using anti-IL-17A/Il17a antibody. IL-17A/Il17a was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL-17A/Il17a Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of IL-17A/Il17a using anti-IL-17A/Il17a antibody. IL-17A/Il17a was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL-17A/Il17a Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Quick Database Links

Gene Symbol

m7GpppN-mRNA hydrolase

UniProt

Q61453

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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