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IL10 Rabbit Polyclonal Antibody
SKU: orb692186
Description
Research Area
Cancer Biology, Immunology & Inflammation
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC |
|---|---|
| Dilution Range | Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat ELISA, 0.1-0.5μg/ml, |
| Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived rat IL10 recombinant protein (Position: K52-A170). |
| Target | Interleukin-10 |
| Molecular Weight | 19 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. |
| Concentration | 500 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF
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IHC analysis of IL10 using anti-IL10 antibody. IL10 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL10 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of IL10 using anti-IL10 antibody. IL10 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL10 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Quick Database Links
Gene Symbol
Interleukin-10
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL10 Rabbit Polyclonal Antibody (orb692186)
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