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IL17A Rabbit Polyclonal Antibody

SKU: orb1184716

Description

Anti-IL17A Antibody. Tested in IHC applications. This antibody reacts with Human.

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsIHC
Dilution RangeImmunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml/ml, Human
ReactivityHuman

Related Conjugates & Formulations

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenA synthetic peptide corresponding to a sequence in the middle region of human IL17A, which shares 88.2% amino acid (aa) sequence identity with mouse and rat IL17A.
TargetGrowth and differentiation factor 9
Molecular Weight27 kDa
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CTLA 8; CTLA8; IL 17; IL 17A; IL17; IL-17; IL17A; interleukin 17A

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IL17A Rabbit Polyclonal Antibody

IHC analysis of IL17A using anti-IL17A antibody. IL17A was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL17A Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

UniProt Details

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Protocol Information

IHC
Immunohistochemistry
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IL17A Rabbit Polyclonal Antibody (orb1184716)

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100 μg
$ 450.00
DispatchUsually dispatched within 2-4 weeks
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