Integrin beta 4/ITGB4 Antibody

SKU: orb180705

Description

Images & Validation

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Item 1 of 5

Tested Applications	FC, ICC, IF, IHC, IHC-Fr, WB
Reactivity	Human
Application Notes	Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunofluorescence, 2μg/ml, HumanImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E.coli-derived human Integrin beta 4 recombinant protein (Position: N28-A266). Human Integrin beta 4 shares 88% and 86% amino acid (aa) sequences identity with mouse and rat Integrin beta 4, respectively.
Molecular Weight	210 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Disclaimer	For research use only

Alternative Names

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Integrin beta-4; GP150; CD104; ITGB4

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Flow Cytometry analysis of A431 cells using anti-ITGB4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of ITGB4 using anti-ITGB4 antibody.Lane 1:human A549 cell;2:human PC-3 cell;3:human T-47D cell;4:human HeLa cell;5:human Caco-2 cell.

IF analysis of ITGB4 using anti-ITGB4 antibody.ITGB4 was detected in paraffin-embedded section of human placenta tissues.

IHC analysis of ITGB4 using anti-ITGB4 antibody.ITGB4 was detected in paraffin-embedded section of human placenta tissues.

IHC analysis of ITGB4 using anti-ITGB4 antibody.ITGB4 was detected in paraffin-embedded section of human intestinal cancer tissues.

Quick Database Links

UniProt

P16144

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-Fr

Immunohistochemistry Frozen

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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