Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human MBD4, identical to the related rat and mouse sequences.
Target	Methyl-CpG-binding domain protein 4
Molecular Weight	80 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Methyl-CpG-binding domain protein 4; 3.2.2.-; Methyl-CpG-binding endonuclease 1; Methyl-CpG-binding protein MBD4; Mismatch-specific DNA N-glycosylase; MBD4; MED1

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Western blot analysis of Lane 1: Rat Brain Tissue Lysate, Lane 2: MCF-7 Cell Lysate, Lane 3: HELA Cell Lysate using MBD4 antibody

Western blot analysis of Lane 1: Recombinant Human MBD4 Protein 10ng, Lane 2: Recombinant Human MBD4 Protein 5ng, Lane 3: Recombinant Human MBD4 Protein 2.5ng using MBD4 antibody

Western blot analysis of Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Kidney Tissue Lysate, Lane 3: A549 Cell Lysate, Lane 4: HELA Cell Lysate, Lane 5: MCF-7 Cell Lysate using MBD4 antibody

Quick Database Links

Gene Symbol

Methyl-CpG-binding domain protein 4

UniProt

O95243

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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