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mCherry Antibody
SKU: orb11618
Featured
Description
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| Tested Applications | IEM, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500 |
| Reactivity | Other |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Antigen: Purified recombinant peptide produced in E. coli.. Antigen Sequence: MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK |
| Target | Red Fluorescent Protein |
| Purification | Epitope affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS, 20% glycerol and 0.05% sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.
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Western blot analysis of transfected 293 HEK with mCherry cell lysate using mCherry antibody
Immunohistochemical analysis of mouse mammary tumor cell line using mCherry goat antibody
Confocal immunofluorescence analysis of COS-7 transfected with mCherry-EEA1 using mCherry antibody
Quick Database Links
Gene Symbol
Red Fluorescent Protein
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
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Esther C H Uijttewaal et al. CRISPR-StAR enables high-resolution genetic screening in complex in vivo models Nat Biotechnol, (2024)
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Olga A Balashova et al. Noncanonical function of folate through folate receptor 1 during neural tube formation Nat Commun, 15(1):, 1642 (2024)
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Y Sun et al. Brain-wide neuronal circuit connectome of human glioblastoma Nature, 641, pages222–231 (2025)
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Talley MJ et al. Distinct requirements for Tcf3 and Tcf12 during oligodendrocyte development in the mouse telencephalon Neural Dev., 18(1), 5 (2023)
Zhou, Tian et al. Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice Nat Commun, 13, 6233 (2022)
Huang, Yubin et al. Repopulated microglia are solely derived from the proliferation of residual microglia after acute depletion Nat Neurosci, 21, 530-540 (2018)
Gong, ChenZi et al. Human spinal GABA neurons alleviate spasticity and improve locomotion in rats with spinal cord injury Cell Rep, 34, 108889 (2021)
Jonscher, Ernst et al. Two COWP-like cysteine rich proteins from Eimeria nieschulzi (coccidia, apicomplexa) are expressed during sporulation and involved in the sporocyst wall formation Parasit Vectors, 8, 395 (2015)
Ramesh, Girish et al. A short isoform of STIM1 confers frequency-dependent synaptic enhancement Cell Rep, 34, 108844 (2021)
Li, Fei et al. Murine leukemia virus Gag localizes to the uropod of migrating primary lymphocytes J Virol, 88, 10541-10555 (2014)
mCherry Antibody (orb11618)
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