Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E. coli-derived mouse Osteocalcin recombinant protein (Position: Y50-I95). Mouse Osteocalcin shares 69% and 68.9% amino acid (aa) sequence identity with human and rat Osteocalcin, respectively.
Target	Osteocalcin
Molecular Weight	29 kDa, 60 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 5mg rAlbumin, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Osteocalcin; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein; Bglap

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Osteocalcin/BGLAP Rabbit Polyclonal Antibody

IHC analysis of Osteocalcin using anti-Osteocalcin antibody. Osteocalcin was detected in paraffin-embedded section of mouse tibia tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Osteocalcin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Quick Database Links

Gene Symbol

Osteocalcin

UniProt

P86546

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

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