PKC delta/PRKCD Rabbit Polyclonal Antibody

SKU: orb234285

Description

Anti-PKC delta/PRKCD Antibody. Tested in IF, ICC, WB applications. This antibody reacts with Human.

Research Area

Cancer Biology

Images & Validation

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Item 1 of 2

Tested Applications	IHC, WB
Dilution Range	Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Reactivity	Human, Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E.coli-derived human PKC delta recombinant protein (Position: M1-E160). Human PKC delta shares 95% and 93% amino acid (aa) sequence identity with mouse and rat PKC delta, respctively.
Target	Protein kinase C delta type
Molecular Weight	78 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Protein kinase C delta type; 2.7.11.13; Tyrosine-protein kinase PRKCD; 2.7.10.2; nPKC-delta; Protein kinase C delta type regulatory subunit; Protein kinase C delta type catalytic subunit; Sphingosine-dependent protein kinase-1; SDK1; PRKCD

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PKC delta/PRKCD Rabbit Polyclonal Antibody

IF analysis of PKC delta using anti-PKC delta antibody. PKC delta was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/mL rabbit anti-PKC delta Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-PKC delta antibody, Western blotting. All lanes: Anti PKC delta at 0.5 ug/ml. Lane 1: HELA Whole Cell Lysate at 40 ug. Lane 2: SMMC Whole Cell Lysate at 40 ug. Lane 3: U87 Whole Cell Lysate at 40 ug. Lane 4: JURKAT Whole Cell Lysate at 40 ug. Lane 5: CEM Whole Cell Lysate at 40 ug. Lane 6: A549 Whole Cell Lysate at 40 ug. Predicted bind size: 77 KD. Observed bind size: 77 KD.

Quick Database Links

Gene Symbol

Protein kinase C delta type

UniProt

Q05655

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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