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Description
Research Area
Cancer Biology, Cardiovascular Research, Metabolism Research, Signal Transduction
Images & Validation
−
Item 1 of 15
| Tested Applications | ELISA, FC, ICC, IF, IHC, WB |
|---|---|
| Dilution Range | Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml |
| Reactivity | Human, Mouse, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human SPHK1 recombinant protein (Position: R16-H267). |
| Target | Sphingosine kinase 1 |
| Molecular Weight | 45-50 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. |
| Concentration | 500 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Hexokinase-2; 2.7.1.1; Hexokinase type II; HK II; Muscle form hexokinase; HK2
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Quick Database Links
Gene Symbol
Sphingosine kinase 1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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Usually dispatched within 2-4 weeks