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TGF beta 1/TGFB1 Rabbit Polyclonal Antibody

SKU: orb1098055

Description

Anti-TGF beta 1/TGFB1 Antibody. Tested in IHC applications. This antibody reacts with Rat.

Research Area

Cancer Biology, Disease Biomarkers, Protein Biochemistry, Signal Transduction

Images & Validation

Tested ApplicationsIHC
Dilution RangeImmunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Rat
ReactivityRat

Related Conjugates & Formulations

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenA synthetic peptide corresponding to a sequence at the N-terminus of human TGF beta 1/TGFB1, identical to the related mouse and rat sequences.
TargetTransforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide
Molecular Weight120 kDa
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CED; DPD1; LAP; TGF beta 1; tgf beta1; TGFB; TGFB1; TGFbeta; TGF-beta 1; tgfbeta1; tgf-beta1; TGFβ; TGF-β; TGFβ1; TGF-β1

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TGF beta 1/TGFB1 Rabbit Polyclonal Antibody

IHC analysis of TGF Beta 1/TGFB1 using anti-TGF Beta 1/TGFB1 antibody. TGF Beta 1/TGFB1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-TGF Beta 1/TGFB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

UniProt Details

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Protocol Information

IHC
Immunohistochemistry
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TGF beta 1/TGFB1 Rabbit Polyclonal Antibody (orb1098055)

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Available Sizes

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100 μg
$ 450.00
DispatchUsually dispatched within 2-4 weeks
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