Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E. coli-derived human TIMP3 recombinant protein (Position: C24-P211). Human TIMP3 shares 97.9% and 97.3% amino acid (aa) sequence identity with mouse and rat TIMP3, respectively.
Target	Metalloproteinase inhibitor 3
Molecular Weight	34 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 5mg rAlbumin, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Metalloproteinase inhibitor 3; Protein MIG-5; Tissue inhibitor of metalloproteinases 3; TIMP-3; TIMP3

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Western blot analysis of TIMP3 using anti-TIMP3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50 ug, Lane 2: Mouse Ovary Tissue Lysate at 50 ug, Lane 3: HELA Whole Cell Lysate at 40 ug, Lane 4: MCF-7 Whole Cell Lysate at 40 ug, Lane 5: SMMC Whole Cell Lysate at 40 ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TIMP3 at approximately 34 kDa. The expected band size for TIMP3 is at 34 kDa.

Quick Database Links

Gene Symbol

Metalloproteinase inhibitor 3

UniProt

P35625

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols