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Apelin Receptor Rabbit Polyclonal Antibody
SKU: orb526574
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Description
Images & Validation
−Item 1 of 3
| Tested Applications | WB |
|---|---|
| Dilution range | WB=1:500-2000 |
| Reactivity | Mouse |
| Predicted Reactivity | Canine, Equine, Gallus, Human, Rabbit, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Apelin Receptor (181-280/380aa) |
| Target | APLNR |
| Molecular Weight | 42 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−APJ receptor; AGTR L1; AGTRL 1; AGTRL1; Angiotensin II receptor like 1; Angiotensin receptor like 1; Apelin receptor; APJ; B78; FLJ90771; G protein coupled receptor APJ; GPCR34; HG 11; HG11; MGC45246; MSR; msr/apj; Aplnr; Agtrl1; APLNR; FLJ96609; APJ_HUMAN.
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Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug, Lane 2: Lung (Mouse) Lysate at 40 ug, Lane 3: Thymus (Mouse) Lysate at 40 ug, Primary: Anti-Apelin Receptor (orb526574) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Apelin Receptor) Polyclonal Antibody, Unconjugated (orb526574) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Lung (Rat) Lysate at 40 ug, Lane 2: Spleen (Rat) Lysate at 40 ug, Primary: Anti-Apelin Receptor (orb526574) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.
Quick Database Links
Gene Symbol
APLNR
UniProt
UniProt Details
− No UniProt data available
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Apelin Receptor Rabbit Polyclonal Antibody (orb526574)
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