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Description
Research Area
Neuroscience
Images & Validation
−
Item 1 of 8
| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human ASCL1 (151-236/236aa) |
| Target | ASCL1 |
| Molecular Weight | 26 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ASH1; HASH1; MASH1; bHLHa46; ASCL1_HUMAN; ASCL1; ASH-1; Class A basic helix-loop-helix protein 46 (bHLHa46); ASCL1_MOUSE; mASH-1; ASCL1_RAT; achaete-scute family bHLH transcription factor 1; achaete-scute complex (Drosophila) homolog-like 1; achaete-scute complex-like 1 (Drosophila); achaete-scute complex homolog 1 (Drosophila)
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Quick Database Links
Gene Symbol
ASCL1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
Mendes-da-Silva, Cristiano et al. Increased expression of Hes5 protein in Notch signaling pathway in the hippocampus of mice offspring of dams fed a high-fat diet during pregnancy and suckling Int J Dev Neurosci, 40, 35-42 (2015)
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