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Beta-Amyloid (1-42) Mouse Monoclonal Antibody
SKU: orb783127
Featured
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:500-5000, IHC-F=1:500-5000, IF=1:200-5000 |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | 6C8 |
| Immunogen | KLH conjugated synthetic peptide derived from human beta-Amyloid(1-42) (672-713/770 or 1-42/42aa) |
| Target | APP |
| Molecular Weight | 4.4 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−beta-Amyloid(1-42); A4; AAA; ABETA; ABPP; AD1; Alzheimers Disease Amyloid Protein; Amyloid B; Amyloid beta A4 Protein Precursor; Amyloid beta; Amyloid of Aging and Alzheimer Disease; APP; APPI; B Amyloid; beta APP; Cerebral Vascular Amyloid Peptide; CTFgamma; CVAP; PN II; PN2; PreA4; Protease nexin II; A beta; Amyloid 1-42.
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100 ng Aβ1-42 Peptide per lane probed with beta-Amyloid (1-42) polyclonal antibody respectively, unconjugated (orb783127) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
Positive sample (left): Alzheimer's Disease Brain, Negative sample (right): Normal Human Brain, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (beta-Amyloid (1-42)) Monoclona Antibody, Unconjugated (orb783127) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Quick Database Links
Gene Symbol
APP
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Beta-Amyloid (1-42) Mouse Monoclonal Antibody (orb783127)
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