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Beta galactosidase Rabbit Polyclonal Antibody
SKU: orb2863
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Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, FC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:1000-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000, Flow-Cyt=1ug/Test |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant human GLB1 protein (329-677/677aa) |
| Target | GLB1 |
| Molecular Weight | 71 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−BGAL_HUMAN; beta-galactosidase; GLB1; EC:3.2.1.23; Acid beta-galactosidase(Lactase); Elastin receptor 1; ELNR1; galactosidase beta 1; EBP; ELNR1; MPS4B; β-Galactosidase; β Galactosidase.
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Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: Liver (Rat) Lysate at 40 ug, Lane 3: U87MG (Human) Cell Lysate at 30 ug, Lane 4: MCF-7 (Human) Cell Lysate at 30 ug, Lane 5: JEG-3 (Human) Cell Lysate at 30 ug, Lane 6: Huvec (Human) Cell Lysate at 30 ug, Primary: Anti-Beta galactosidase (orb2863) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD.
U87MG cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Beta galactosidase) polyclonal Antibody, Unconjugated (orb2863) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Quick Database Links
Gene Symbol
GLB1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Beta galactosidase Rabbit Polyclonal Antibody (orb2863)
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